Coding

Part:BBa_J51002:Design

Designed by: Peter Nguyen   Group: iGEM06_Rice   (2006-10-25)

ComQX


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

NOTE: The Biobrick prefix and suffix sites are non-standard! Due to the high percentage of A+Ts in the ComQXPA genes, the primer hybridization sites were lengthened and the Biobrick sites correspondingly shortened (the NotI sites between the E-X and S-P restriction sites was removed).

Primers used for isolation and construction (5'->3')

F: cggaattccctctagATGAAGGAGATTGTGGAGCAAAACATATTTAACG

R: gactgcagctactagTTAATCACCCCATTGACGGGTTATTGG

Source

This part was PCR amplified from Bacillus subtilis 168 genomic DNA.

References